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To grow

Both forms must be protected from light, moisture, and repeated temperature cycling

But there are several obstacles to the development of this senolytic approach such as the long-term effect of CAR-T cells therapy with possible aspecific toxicity in different organs 56 , the cost and the difficulty during process development 57 or the selection of non-exhausted T cells to generate efficient therapy 58,59

The DOD have provided videos to help you through this process which can be found here