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Cells were routinely cultured in Dulbeccos modified eagle medium (DMEM) (MT10013CV, Fisher Scientific,) with sodium pyruvate, supplemented with 10% fetal bovine serum (FBS) (13206C, Sigma-Aldrich), 1% penicillin and streptomycin (P/S) (15140122, Fisher Scientific), 5 g/mL plasmocin (ant-mpp, Invivogen), and cultured in a humidified incubator with 5% CO 2 at 37 C

Some people also have an allergic reaction that may involve hives, swelling, and difficulty breathing, though it is rare

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On the other hand, there may be negative consequences if ethylene levels rise too high

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