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Description

Metallomics 9 (12), 17031734

Mechanisms and significance of eryptosis, the suicidal death of erythrocytes

The samples were thawed on ice, centrifuged at 15,700 RCF for 5 min at 4 C and the supernatant was collected for downstream processing

Protein was determined spectrophotometrically at 595 nm based on the Bradford method 29

Tissue was homogenized by probe sonication (2 10 pulses, 40% power output), and particulate matter was removed by passing samples through 0.4 m syringe filters