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Because the reconstitution protocol only matters if the peptide you're starting with is molecularly sound

At low stoichiometries, 1:1 binding dominated, whereas higher stoichiometries will resulted in increasing amounts of 1:2 and eventually 1:3 binding (peptide:Cu)

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In a similar fashion, the pyrimidine biosynthesis inhibitor Gemcitabine also impaired the replication of HEV through the activation of STAT1 and the induction of ISGs through a non-canonical pathway (188)

The p38 MAPK/JNK pathways play a central role in stress response and cell death, and their inhibitors can significantly attenuate epithelial apoptosis[42, 43]

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