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doi: 10.1210/jc.2016-2660 40 De CastroJPWFonsecaTLUetaCBMcAninchEAAbdallaSWittmannGet al

Real-time quantitative reverse transcription PCR (RT-qPCR) was performed with the TB Green Premix Ex Taq kit (RR420W, Takara, Japan) on an ABI 7500 PCR instrument (Applied Biosystems, USA)

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Studies have shown that excessive activation of the Wnt/-catenin pathway suppresses tumor immune surveillance by upregulating immune evasionrelated factors, such as PD-L1 and CD47, thereby enabling tumors to escape host immune responses (76)