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Constitutive activation of NF-kappaB during progression of breast cancer to hormone-independent growth

The protocol for immobilization of GST-SPINK3 to glutathioneagarose beads was modified from previously reported protocols by using an alternative bifunctional cross-linker (dithiobis(succinimidyl propionate)) in a very simple procedure and by using simple buffers under physiological conditions

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This approach allows for the functional characterization of key genes and the exploration of their therapeutic potential