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MS tumor tissues were mechanically lysed in RIPA buffer supplemented with a protease inhibitor cocktail, and the supernatant was collected by centrifugation for protein quantification using a BCA assay

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at the same time, researches show that GCH1 has specific competitive antagonism with ferroptosis (Wang Z

All mice were enrolled in the study in numerical order and randomly allocated to experimental groups by lottery, with the group allocation performed by a third-party investigator to ensure that the experimenters remained blinded to group assignments

2016;11:e0155694

0.48, 0.62 and 12.7 mg/ dL and 25.4%, respectively)