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The eluted sample was incubated at 65 C overnight to reverse the crosslinking, and DNA was extracted using a TIANquick Midi purification kit (TIANGEN, DP204, Beijing, China) and subjected to PCR analysis

These lines of evidence and decision-making tools can be used to identify priorities and next steps to advance a given technology, assess whether a technology is ready for field demonstration, and identify key areas of uncertainty regarding technology performance

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