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The erythrocytes were then lysed with lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, USA) for 10 min followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and resuspension of the cells in Hepes-buffered saline (HBS, 14 mM Hepes, 0.9% NaCl)

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In contrast, a negligible difference in GFP - ACA11-F3 half-life was observed when it was co-expressed with either TZF1 or TZF1 H186Y , in agreement with our observation of specific binding of TZF1 to the F1/F4 region of ACA11 in RIP-qPCR experiments ( Figures 6D, E )

[40] [41] Their next album would be recorded with producer John Feldman

Ferroptosis occurred at an early stage of lung disease and accompanied disease progression with high spatial correlation at lung lesion sites

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