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The lack of carcinogenic activity of 2-hydroxyestrogens has been attributed to a high rate of clearance, more rapid rate of O -methylation and lower hormonal potency in estrogen target tissues, plus produces 2-methoxyestradiol, which suppress tumor cell proliferation and angiogenesis [19]

Additionally, all experimental procedures involving animals were performed in accordance with the institutional guidelines and regulations for the care and use of experimental animals and complied with the relevant national ethical standards

doi: 10.1016/j.fct.2023.113726 85 ZouZ.HuW.KangF.XuZ.LiY.ZhangJ.et al

34 Furthermore, a study reported that severe 25[OH]D deficiency was accompanied by an increase in TNF- in diabetic patients suffering from painful peripheral neuropathy

The pathway begins in the mitochondrial matrix with the rate-limiting reaction catalyzed by 5-aminolevulinic acid synthase (ALAS), a PLP-dependent homodimeric enzyme that condenses succinyl-CoA and glycine to produce 5-aminolevulinic acid (ALA), releasing CO and CoASH

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