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After drying, a 100 l sample diluent was added to each well and incubated on a shaker at room temperature for 1 h to block the protein chip

They work best when integrated into a broader approach that includes proper nutrition, adequate rest, stress management, movement, and other foundations of vitality

Disclosure and scope

Presentation of Graves orbitopathy within European Group On Graves Orbitopathy (EUGOGO) centres from 2012 to 2019 (PREGO III)

After 96 h, wells were aspirated, washed with PBS, and a fixative/staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399) was added