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We used primary antibodies for GPx4 (Abcam, ab125066), acrolein (JaICA, MAR-020n), cleaved caspase substrate motif (Cell Signaling Technology, 8698), COX IV (Cell Signaling Technology, 4844), FLAG (Cell Signaling Technology, 2368), cytochrome c (Cell Signaling Technology, 11940), RIP3K (Cell Signaling Technology, 15828), heme HO-1 (Enzo Life Sciences, ADI-SPA-895), Top2b (Abcam, ab125297), GAPDH (Santa Cruz Biotechnology Inc., sc-32233), and -actin (Santa Cruz Biotechnology Inc., sc-47778)

10.1139/cjpp-2020-0302 11 EchtayK

Nature 485, 512516

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For long-term storage of reconstituted material, aliquot into single-use volumes and freeze at 80 C to avoid repeated freeze-thaw cycles, which are the single largest source of variability in peptide assays

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