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In most cells, 1C metabolism exhibits a directional flux from mitochondria to cytosol [47, 48]

All lenses were incubated for 3 to 4 days in 2 mL of M199 medium (Sigma-Aldrich) containing 0.1% BSA and 30 mM Galactose using an incubator set at 5% CO 2 and 37 C to induce opacity, as previously reported 30

The condition's incidence has accelerated due to increased UV exposure from reduced ozone protection in equatorial regions, higher altitude living, and greater time spent outdoors for work and recreation

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However, D4 also shows off-target effects blocking other connexin channels, including Cx26, Cx30 and Cx45 522,523