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In the final step to the coenzyme, CobC removes the 5' phosphate group: [32] [33] Adenosylcobalamin-5'-phosphate + H 2 O adenosylcobalamin + phosphate The complete biosynthetic route involves a long linear path that requires about 25 contributing enzyme steps

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We recognize the critical need for future studies to validate these using human-derived cell lines and PDX models to confirm the translational significance of our results, and are developing and testing strategies for utilizing immunodeficient mice for studies of obesity and cancer [40]