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Equal amounts of cell protein lysates were separated by SDS PAGE and transferred to PVDF membranes (Millipore, Darmstadt, Germany)

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Blank was the reaction mixture, and positive control was the reaction mixture illuminated in light for the same time, without extract

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Since no other cause for the metabolic acidosis could be found, the possibility of accumulation of 5-oxoproline by paracetamol and flucloxacillin was recognised and the treatment with paracetamol was stopped

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