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Anergic T cells are metabolically anergic

The anterior segment, the lens and the retina were removed, and the RPE/choroid tissue (posterior eye) was treated with 0.5% Triton X100 in 5% blocking buffer (with blocking serum in which the secondary antibody was raised) and subsequently used for immunolabeling with antiCD31 antibodies to detect blood vessels, Alexa488conjugated phalloidin, and antigalactosidase antibody to confirm lacZ expression in the RPE as a genotype control

Metabolic characterization of imatinib-resistant BCR-ABL T315I chronic myeloid leukemia cells indicates down-regulation of glycolytic pathway and low ROS production

H., Kim S

Ressurreio M, Elbeyioglu F, Kirk RS, Rollinson D, Emery AM, Page NM, et al

The greyscales of the bands were measured using the ImageJ software for further analysis