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recently detected five genetic variants related to age at onset in 5,462 asthma cases, at or around recombinant Cylindromatosis (CYLD) on 16q12 (rs1861760), IL1RL1 on 2q12 (rs10208293), HLA-DQA1 on 6p21 (rs9272346), IL33 on 9p24 (rs928413) and GSDMA on 17q12 (rs9901146), with the last four also showing associations with susceptibility to allergic diseases

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RNA concentrations were determined by OD measurement at 260 nm on a spectrophotometer (Implen), and cDNA was synthesized from the purified RNA by both random and oligo (dT) priming using an iScript cDNA synthesis kit (TIANGEN)

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TRPM7 is overexpressed in human IBD-related and sporadic colorectal cancer and correlates with tumor grade