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doi: 10.1002/hep.20437

Materials and methods Cell culture Human acute monocytic leukemia cells, THP1, and the mouse leukemic monocyte cell line, RAW264.7, were cultured using RPMI-1640 medium modified to exclude calcium nitrate and supplemented with 2.05 mM L-Glutamine (Gibco, C11875500BT), 10% fetal bovine serum (FBS) (ExCell, FSP500), and a 1% penicillinstreptomycin mixed solution (Abiowell, AWH0529a)

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In another pilot study, 15 patients with melanoma metastases to the liver who underwent cryoablation and pembrolizumab experienced persistently increased serum IL-6 levels immediately after cryoablation (88)