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While CellROX Orange is not suitable to distinguish and quantify specific forms of ROS, in this study it is used to estimate the overall oxidative stress response of a cell

The peptide is often sold through unregulated channels as a 'research chemical' or unauthorised medicine, raising significant concerns about purity, dosage accuracy, and contamination

Following a washing step, Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:400, Molecular Probes, Waltham, MA, USA) and/or Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400, Molecular Probes) were/was added as the secondary antibody and DAPI was used for nuclear staining

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