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Yes, B12 can expire

Roche, Grenzach-Wyhlen, Germany) was mixed with 1 mL Dulbeccos Modified Eagles Medium (DMEM

10.1016/j.crohns.2010.05.010 350

13 C 6 -Glucose tracing metabolomics Cells were cultured in complete NB medium (1 10 6 cells/T-25 flask), without D-glucose supplemented with 4.5 g/L 13 C 6 -glucose (Sigma) and incubated at 37 C for 24 h