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Ribosomal protein S6 kinase 1 signaling regulates mammalian life span

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To identify the specific cell types producing occludin, breast milk-treated monolayers were co-stained to detect the presence of occludin as well as lysozyme, a marker for Paneth cells, trefoil factor 3 (TFF3), a marker for goblet cells, and chromogranin A, a marker for enteroendocrine cells

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