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the invention encompasses a method of providing muscular therapy by administering a cation of Formula I, wherein: R 1 is C 1-4 alkyl R 2 , R 3 , R 4 , and R 5 are independently selected from the group consisting of: H, C1-4 alkyl, halogen-substituted C 1-4 alkyl, NR 9 R 10 , and CN

Nutritional Approaches to Neurodegenerative Diseases, presented at Functional Medicine 98

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Cell lysates were collected in cell lysis buffer (20 mM Tris, 0.5 mM dithiothreitol, 150 mM NaCl, 0.5% Triton X-100, 2 g/mL pepstatin, 2 g/mL bestatin, 2 g/mL leupeptin, 4 mM phenylmethylsulfonyl fluoride, 10 g/mL aprotinin, 1 mM tetra-sodium pyrophosphate decahydrate, 10 mM -glycerophosphate, 1 mM sodium orthovanadate, 1 M sodium fluoride, pH 7.5)