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Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT

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An intravenous infusion is defined as the delivery of fluids through a vein using a needle or similar device

Benefits of Toradol Injections Anti-Inflammatory Toradol helps reduce inflammation at the site of injury or pain, alleviating swelling, redness, and discomfort associated with inflammatory conditions

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The inclusion of 0.9% benzyl alcohol provides antimicrobial preservation, helping maintain solution integrity following repeated vial access when appropriate aseptic laboratory techniques are utilized