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Indeed, while the 21mer Thr1273Glu mutant S tail binds to WD40 domains more tightly than the 21mer wild-type S tail, its binding kinetics are substantially slower than of the wild-type S tail 21mer (Fig

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2.6 Measurement of ROS levels ROS generation was determined with the fluorescent ROS probe 2,7-dichlorodihydrofluorescein diacetate (H 2 DCF-DA)

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