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Furthermore, a combination of plant extracts resulted in a higher inhibition of collagenase activity under in vitro conditions

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The solvent gradient (A/B/C) used for eluting estrogen metabolites is as follows: 8 min of isocratic at an initial composition of 16/68/16, 7 min of a concave gradient (curve number 9) to 18/64/18, 13 min of a concave gradient (curve number 8) to 20/59/21, 10 min of a convex gradient (curve number 2) to 22/57/21, 13 min of a concave gradient (curve number 8) to 58/21/21, followed by a 0.1-min step to 92/5/3 and a 8.9-min isocratic period at 92/5/3