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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol

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Celecoxib alleviates AKT/c-Met-triggered rapid hepatocarcinogenesis by suppressing a novel COX-2/AKT/FASN cascade

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Accumulation of these compounds can result in oxidative stress, which occurs when the generation of free radicals in the body exceeds the bodys ability to neutralize and eliminate them