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xylostella , including ALP and ABCC, are regulated by the MAPK signal pathway, and a high level of hormones promotes the expression of ALP and ABCC by activating the MAPK pathway, leading to Cry resistance (19)

After 48 h of incubation at 27 C, cells from 10 mL of culture were harvested by centrifugation at 300 g for 5 min at 4 C

Total protein was quantified using a Pierce BCA Protein Assay kit (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol

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Iron Women with insufficient iron levels may experience anovulation, where the ovaries fail to release a mature egg

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