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While elevated systemic BCAA levels were correlated with increased atherosclerosis risks, the direct role of BCAA metabolism within ECs during atherogenesis, particularly with aging, remains unclear

In another method, Soxhlet extraction was performed using n -hexane solvent for nine hours at 70 C and a solid-to-solvent ratio of 1:10

These primers were employed to amplify the zeo-PheS cassette from the pR6K-attL-zeo-phes-attR2 plasmid

Jain, V

2.2 Total protein concentration Protein concentration was measured using Bradford's (1976) method, which relies on the interaction of protein with Coomassie Brilliant Blue G250 in acidic conditions

Methods Bone marrow-derived MSC exosomes (Que-Exo) were isolated after preconditioning with quercetin (1M, 24 h)