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Frosty container, placed at 80 C overnight to complete cryopreservation, and submitted to Active Motif for ATAC-sequencing

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In addition, AMPK also triggers activation of JNK, which in turn phosphorylates the scaffolding protein Lim domain-only 7b (LMO7b)

Anti-Doping Agency (USADA) 9

In terms of experimental design and in order to limit possible confounding effects due to variability between litters or genetic differences across strains, experiments were designed to include animals from multiple litters, typically three independent litters per group and littermate WT animals used as controls 63