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The cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin G, and 100 g/ml streptomycin at 37C in a humidified 5% CO2-95% air incubator under standard conditions

Two conserved cysteines of HIV- 1 protease (Cys-67, Cys-95) are readily glutathionylated, resulting in an enzyme preparation with significantly lower specific activity