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Additionally, as chelating agents have a rebound effect, they cannot be used in instances where lead exposure cannot be suspended

The slides were incubated with blocking buffer for 30 min at 37 C, followed by incubation overnight with anti-human LC3 antibody at 4 C and then Alexa Fluor 488 goat anti-rabbit IgG at a 1:1000 dilution at ambient temperature for 1 h