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Also valine has been proven important for the oxidation of the reduced flavin by molecular oxygen in choline oxidase and it has been reported that replacement of Val464 with alanine in the enzyme results in a twofold decrease in the limiting rate constant for flavin reduction (k red ) and less than fivefold decrease in the equilibrium constant for formation of the enzymesubstrate complex (K d ) 52

Multiple small clinical trials have explored its effects on mood, with some finding results comparable to certain antidepressants.8 At 200mg per serving, the dose reflects amounts commonly used in research

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