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In spinal cord tissue from both sporadic and familial ALS patients, researchers have reported a marked depletion of GPX4 [235], indicating an impaired ability to detoxify lipid peroxides

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doi:10.1172/JCI137558 4

The two analyses identified almost identical sets of genes.) Examining the up- and downregulated genes, we found that 23 genes were downregulated (7.84 to 2.71 fold) and only two were upregulated (4.55 to 2.48 fold) (Table 2)

Tissues were then put into 0.15 M sodium cacodylate pH 7.4 containing 2.5% glutaraldehyde and fixed for additional 20 min at 4 C