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To establish the baseline of IGF-I binding to IGFBP-3, we incubated IGFBP-3 in a range of concentrations of IGF-I (0.5, 2, 10, 50 and 100 g/ml) in phosphate buffer saline (PBS, pH 7.4), giving a final IGF-I:IGFBP-3 molar ratio of 0.01, 0.1, 1, 5 and 10:0.1

DOI: 10.1007/s13311-017-0582-3

Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & -mercaptoethanol (35 mM)

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A number of studies have indicated the existence of a nuclear GSH pool that resists depletion after exposure of cells to BSO (for a review see [42]

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