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The typical regimen involves: Loading dose for patients without neurological involvement : Hydroxocobalamin 1mg intramuscularly three times weekly for two weeks [13] [10] Loading dose for patients with neurological involvement : Hydroxocobalamin 1mg on alternate days until no further improvement, then Maintenance therapy : 1mg every two to three months (or every two months if neurological involvement) Lifelong maintenance therapy is necessary for irreversible causes such as pernicious anaemia or following gastric surgery

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Apparatus UV-visible adsorption spectra were recorded on a U-3900H UVVis Spectrophotometer (Hitachi, Japan) at room temperature using a 500-L black-body quartz curette with 1-cm path length

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