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Comprehensive Evaluation of Lipid Nanoparticles and Polyplex Nanomicelles for Muscle-Targeted mRNA Delivery

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Mettl9 KO cell line validation and off-target analysis by long read sequencing KO To characterise the biallelic deletions in the Mettl9 locus we extracted the genomic DNA from the two Mettl9 KO clones used in the study (#88 and #90) and their parental E14Tg2a cell line with Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs, # T3050L) and performed long-read whole-genome sequencing

Transmission electron microscopy (TEM) The morphology of the particles was studied using HR-TEM, as illustrated in Fig

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Figure 8: torontokangenwater