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Cells were seeded in 24-well plates and co-transfected with PARN-WT or PARN-MUT reporter plasmids together with a Renilla luciferase plasmid as an internal control, using Lipofectamine 3000 (Thermo Fisher Scientific)

doi: 10.1007/s11103-005-4844-9

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F-box and leucine-rich repeat protein 5 (FBXL5) is an E3 ubiquitin ligase that regulates cellular and systemic iron homeostasis by mediating iron regulatory protein 2 (IRP2) degradation (Ruiz and Bruick, 2014)

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