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To generate UBL-fused Eos constructs, FAT10Eos and FAT10Eostail vectors were used as templates for PCR amplification, whereby FAT10 was removed and subsequently replaced by HiFi assembly with either the NUB1 UBL domain (amplified from the full-length codon-optimized NUB1 sequence) or NEDD8 (ordered as dsDNA from Integrated DNA Technologies, codon-optimized for E