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This level of specificity cannot be attained with any other bioanalytical technique employed for biomarker analysis

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Afterwards, 500 ng ml 1 of biotin-conjugated anti-IgE detection antibodies (553419, clone R35-118, BD Biosciences) was incubated for 1 h at room temperature followed by another incubation with diluted HRP-conjugated streptavidin (554066, BD Biosciences) for 30 min at room temperature

doi: 10.1016/j.mjafi.2015.07.002

Surface biotinylation All steps were carried out on ice to prevent surface protein internalisation

Industry sources claim initial results appear after 4-6 IV sessions, typically administered over 2-3 weeks at standard frequency