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Intact cells (OD 600 = 1.0) were resuspended in simulated gastric juice (SGJ:25 mM NaCl, 7 mM KCl, 45 mM NaHCO 3 , 3 g/l pepsin, pH 3.0) and incubated at 37C for 3 h at 200 rpm

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This condition is called Posterior Vitreous Tear or Detachment