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Freeze-dried Powder: Storage Heat: -20C (freezer) Shelf life: 2-3 years when properly stored Protection: Keep away from light and moisture Mixed Solution: Storage Heat: 2-8C (refrigerator) Shelf life: Up to 30 days Protection: Protect from light, use amber vials if available Handling: Avoid repeated heat fluctuations Frozen Aliquots: Storage Heat: -20C or -80C Shelf life: Up to 6 months Protocol: Prepare single-use aliquots to avoid freeze-thaw cycles Thawing: Thaw in refrigerator, use immediately after thawing Safety Monitoring in Research Research protocols involving IGF1 LR3 should include appropriate tracking to ensure subject safety and data quality

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4 The role mechanism of intestinal microbiota and their metabolites in the regulation of pulmonary infection 4.1 Gut bacteria-derived Metabolites Metabolites serve as the critical mediators of the interaction (crosstalk) between the gut microbiota and the human body

doi: 10.1016/j.mib.2019.06.002 105 NaseemS.MinK.SpitzerD.GardinJ.KonopkaJ

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Mayo is an Assistant Clinical Professor of Medicine at Weill Cornell Medical College and an Attending Physician at New York-Presbyterian Hospital and Hospital for Special Surgery