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Similarly, in vitro binding assay with recombinant GSTO1 was performed using 1 M GSTO1 in reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA and 1 M DTT) incubated with compounds for 30 min at 37 C and with CMFDA (500 nM) for an additional 30 min

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Strengths and limitations This systematic review has several strengths in its methodology that enable us to assess the existence of disease biomarkers and the biological processes most likely to be affected by epigenetic deregulation