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Blood 103(10):39053914 Somers K et al (2020) Potent antileukemic activity of curaxin CBL0137 against MLL-rearranged leukemia

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3 Results 3.1 Levels of total, reduced and oxidized glutathione in the heart tissue The analysis of total glutathione content showed no difference between the heart of old and adult rats (Figure 1A)

Post-trypsinization, cells were hypotonically treated with 75 mM KCl for 5 min and stopped by 1 PBS quick wash, then pre-extracted with 0.1% Triton X-100 for 2 min, then washed once by 1 PBS, followed by 4% PFA fixation for 10 min