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For tissue-specific knockout of akt1 in GABAergic neurons, a tissue-specific CRISPR vector ( gad1b:Cas9-T2A-mCherry, U6:gRNA akt1 ) was applied 32,33 , which contains two key promoters: one is the zebrafish U6 promoter, which can drive the expression of the guide RNA (gRNA) akt1 , and the other is the zebrafish gad1b regulatory element, which tissue-specifically controls the expression of zebrafish codon-optimized Cas9 and the fluorescent reporter mCherry (Fig

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