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doi: 10.3390/cancers16040713 71 GeXLiMYinJShiZFuYZhaoNet al

Due to the direct binding of Asn35 to EA, the IC 50 value of N35D was 10-fold greater than that of WT, whereas the IC 50 values of N35A and N35Q were 70- and 160-fold greater than those of WT (Table 1), indicating that the structural specificity of the side-chain of Asn35 is crucial for EA binding

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