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As sensitive biomarker for the evaluation of oxidative stress, GPx is a landmark in the indication of the therapeutic combination of antioxidants until the normalization of this enzyme and the reduction of electrochemical processes leading to cardiac arrhythmias in young patients, and also until the decreased risk of early lipid oxidation (normal or dyslipidemic profile) and for the prophylaxis of early atherosclerotic endothelial processes (27-32)

Neuron 105 , 10271035 e1022 (2020)

doi: 10.1126/science.1224396 76 HaiserH

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Ph1-FLAG and Ph1 phage particles (~ 10 11 ) were reduced with DTT (1 mM) for 30 min at 37 C in a total volume of 100 L

It's a more laborious and expensive process, but it allows for an unparalleled level of quality control