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Coverslips were washed for 3 5 min in Duolink Wash Buffer A and then incubated in 40 L of ligase (Sigma Aldrich, DUO82027) diluted in 1X ligation buffer (Sigma Aldrich, DUO82009) at a 1:40 dilution at 37 C for 30 min

Quantitative real-time PCR The total RNA was extracted from cells or tissue using TRIzol reagent following the manufacturers instructions and reverse-transcribed to cDNA using a ABScript III RT Master Mix for the qPCR Kit (ABclonal)

Chemico-Biological Interactions, 363 , 110019

Movilizacin de cidos grasos: estimula la liberacin de grasas almacenadas (triglicridos) para que sean utilizadas como energa

And granted there may be statistically more complications, but that's a really artificial kind of designation, right

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