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For making them enzyme-responsive, specifically for cancer treatment, structures that get destroyed under overexpression of tumor-associated enzyme matrix metalloproteinase (MMP2, MMP9, MMP13) and cathepsin B are preferred 63

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Conjugation reactions usually involve metabolite activation by a highenergy intermediate and have been classified into two general types: type I (e.g., glucuronidation and sulfonation), in which an activated conjugating agent combines with substrate to yield the conjugated product, and type II (e.g., amino acid conjugation), in which the substrate is activated and then combined with an amino acid to yield a conjugated product (Hodgson, 2004)

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The degree of PP2A methylation is subject to changes according to metabolism