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Then, all animals were euthanized by bleeding after transection of the abdominal vena cava under anesthesia and the liver, heart, soleus muscle, gastrocnemius muscle, tibialis anterior muscle, extensor digitorum longus muscle, epididymal white adipose tissue, and brown adipose tissue were harvested and immersed in RNAlater solution (Qiagen, Venlo, Netherlands) for qPCR analysis

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Notably, the P values for these genes were substantially more significant, and the fold changes in their differential expression levels were greater in the combination group than those in the single-natural product groups (Fig

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