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Blood was collected in a microcapillary tube and the volume measured

The inflammatory mediators can be released both from tumor and host cells and are frequently elevated in cancer cachexia

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Subsequently, Matsuzawa et al

Instead of spray drying, the solution can be concentrated by evaporation at reduced atmospheric pressure (for example, by evaporation at reduced pressure on a rotary evaporator) at temperatures of less than about 70 C., and the resultant solid residue further treated by a purification treatment/scheme in a suitable solvent